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Mouse CD16
SKU: orb2994204
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | BLI: Loaded Anti-Human CD28 mAb(Cat#GMP-A063) on Protein A Biosensor, can bind Mouse CD16-His(Cat#orb2994204) with an affinity constant of 0.22 uM as determined in BLI assay. Loaded Recombinant Mouse IgG2a Fc (Cat#orb2993941) on AMC Biosensor, can bind Recombinant Mouse CD16 (C-6His) (Cat#orb2994204) with an affinity constant of 0.16 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Mouse IgG2b Fc (Cat#orb2993940) on AMC Biosensor, can bind Recombinant Mouse CD16 (C-6His) (Cat#orb2994204) with an affinity constant of 0.121 uM as determined in BLI assay. (Regularly tested) Loaded Recombinant Mouse IgG3 Fc (Cat#orb2993939) on AMC Biosensor, can bind Recombinant Mouse CD16 (C-6His) (Cat#orb2994204) with an affinity constant of 0.62 uM as determined in BLI assay. (Regularly tested) |
| Tag | C-6His |
| Molecular Weight | Predicted: 22 KDa. Observed: 35-40 KDa, reducing conditions |
| Expression Region | Ala31-Thr215 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 10% Sucrose, 8% Mannitol, 0.05% Tween 80, pH 7.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Low affinity immunoglobulin gamma Fc region receptor III; Fcgr3; Fc gamma Receptor III; CD-antigen 16; CD16; FcRIII; IgG Fc receptor III
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Mouse CD16 (orb2994204)
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