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Recombinant Mouse CD52 Protein
SKU: orb2992870
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | ELISA: Immobilized Human CD52 mAb (Cat#GMP-A052) at 10 μg/ml (100 μl/well) can bind Recombinant Mouse CD52 (C-Fc) (Cat#orb2992870)*. *: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Mouse CD52 (C-Fc) (Cat#orb2992870) is 3.82 μg/ml. (Regularly tested). |
| Tag | C-Fc |
| Molecular Weight | Predicted: 29.3 Kda. Observed: 35-50 KDa, reducing conditions |
| Expression Region | Gly24-Ser47 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4 |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CDw52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5 (He5); CD52; CDW52; HE5
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 28.3 kDa after removal of the signal peptide. The apparent molecular mass of CD52-mFc is approximately 33-53 kDa due to glycosylation.
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10 μg, 100 μg, 50 μg Recombinant Mouse CD52 Protein, N-His-SUMO [orb3153051]
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>90% as determined by SDS-PAGE.
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Recombinant Mouse CD52 Protein (orb2992870)
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