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Recombinant Murine Acidic Fibroblast Growth Factor
SKU: orb1906178
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 x106 IU/mg in the presence of 10 μg/ml of heparin. |
| Molecular Weight | Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 140 amino acid residues. |
| Protein Sequence | FNLPLGNYKK PKLLYCSNGG HFLRILPDGT VDGTRDRSDQ HIQLQLSAES AGEVYIKGTE TGQYLAMDTE GLLYGSQTPN EECLFLERLE ENHYNTYTSK KHAEKNWFVG LKKNGSCKRG PRTHYGQKAI LFLPLPVSSD |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1 EU/ug of rMuaFGF as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Disclaimer | For research use only |
Alternative Names
−FGF-1, HBGF-1
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Recombinant Murine Fibroblast Growth Factor-basic (rMubFGF ) [orb1495073]
>98% by SDS-PAGE and HPLC analyses.
Approximately 16.2 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.
Escherichia coli.
1 mg, 50 μg, 10 μg
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Recombinant Murine Acidic Fibroblast Growth Factor (orb1906178)
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