Recombinant Murine Interleukin-10 (rMuIL-10)

SKU: orb1495011

Description

IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNFα. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells.

Images & Validation

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Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Key Properties

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Source	Escherichia coli.
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent co-stimulation (with IL-4) of the proliferation of murine MC/9 cells was found to be 5 x 105 units/mg.
Molecular Weight	Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 161 amino acids
Protein Sequence	MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S
Purification	>97% by SDS-PAGE and HPLC analyses.
Purity	>97% by SDS-PAGE and HPLC analyses.
Endotoxins	Less than 1EU/mg of rMuIL-10 as determined by LAL method.

Storage & Handling

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Storage	This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Form/Appearance	Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
Buffer/Preservatives	Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
Disclaimer	For research use only

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