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Recombinant Murine Leukemia Inhibitory Factor
SKU: orb1906185
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Description
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 x108 IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures. |
| Molecular Weight | Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids. |
| Protein Sequence | SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1 EU/ug of rMuLIF as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % TWEEN 20. |
| Disclaimer | For research use only |
Alternative Names
−Differentiation-stimulating Factor, D factor, Melanoma-derived LPL Inhibitor , MLPLI
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Recombinant Murine Leukemia Inhibitory Factor (orb1906185)
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