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Recombinant Murine Macrophage Colony Stimulating Factor
Description
Images & Validation
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x105 IU/mg. |
| Molecular Weight | Approximately 52.0 kDa, a disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains. |
| Protein Sequence | KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1 EU/ug of rMuM-CSF as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
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| Form/Appearance | Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.4. |
| Disclaimer | For research use only |
Alternative Names
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>98% by SDS-PAGE and HPLC analyses.
Recombinant rat GM-CSF is a 14.5 kDa globular protein consisting of 128 amino acids residues.
Escherichia coli.
1 mg, 20 μg, 5 μgRecombinant Murine Granulocyte- Macrophage Colony Stimulating Factor (rMuGM-CSF) [orb1494926]
>95% by SDS-PAGE and HPLC analyses.
Recombinant murine GM-CSF is a 14.2 kDa globular protein consisting of 124 amino acids residues.
Escherichia coli.
1 mg, 100 μg, 5 μgRecombinant murine Granulocyte- Macrophage Colony Stimlating Factor (rm GM-CSF) [orb1631609]
> 98 % by SDS-PAGE and HPLC analyses.
Recombinant murine GM-CSF is a 14.1 kDa globular protein consisting of 124 amino acids residues.
Murine
20 μl

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Protocol Information
Recombinant Murine Macrophage Colony Stimulating Factor (orb1906186)
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