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Recombinant Murine TNF-related apoptosis-inducing Ligand/TNFSF10
SKU: orb1906183
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Description
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x106 IU/mg in the presence of actinomycin D. |
| Molecular Weight | Approximately 20.2 kDa, a single non-glycosylated polypeptide chain containing 175 amino acids. |
| Protein Sequence | MPRGGRPQKV AAHITGITRR SNSALIPISK DGKTLGQKIE SWESSRKGHS FLNHVLFRNG ELVIEQEGLY YIYSQTYFRF QEAEDASKMV SKDKVRTKQL VQYIYKYTSY PDPIVLMKSA RNSCWSRDAE YGLYSIYQGG LFELKKNDRI FVSVTNEHLM DLDQEASFFG AFLIN |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 0.1 EU/ug of rMuTRAIL/TNFSF10 as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 3 mM DTT. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Recombinant Murine TNF-related apoptosis-inducing Ligand/TNFSF10 (orb1906183)
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