Recombinant Murine Vascular Endothelial Growth Factor 120, Yeast

SKU: orb1906168

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Description

Recombinant Murine Vascular Endothelial Growth Factor 120, Yeast

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Key Properties

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Source	Yeast
Expression System	Yeast
Biological Activity	Fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1-4 ng/ml.
Molecular Weight	Theoretically as a disulfide-linked homodimeric protein, the product consists of two 121 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 20.7 kDa in SDS-PAGE under reducing conditions.
Protein Sequence	MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R
Purity	> 95 % by SDS-PAGE and 90% by SEC-HPLC analyses.
Endotoxins	Less than 0.1 EU/ug of rMuVEGF120, Yeast as determined by LAL method.

Storage & Handling

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Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
Form/Appearance	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Disclaimer	For research use only

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