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NCL Antibody
SKU: orb1410331
Description
Images & Validation
−Item 1 of 9
| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | NCL/7014R |
| Immunogen | Recombinant full-length human NCL protein |
| Target | NCL |
| Source/Expression System | HEK293 cells |
| Molecular Weight | 76kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Disclaimer | For research use only |
Alternative Names
−NCL; Nucl; Nucleolin; Protein C23
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Formalin-fixed, paraffin-embedded cat liver stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2 ug/ml. HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.
Formalin-fixed, paraffin-embedded cow heart stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2 ug/ml. HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.
Formalin-fixed, paraffin-embedded horse lung stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2 ug/ml. HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.
Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R).
Formalin-fixed, paraffin-embedded human kidney stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R).
Formalin-fixed, paraffin-embedded human salivary gland stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.
Formalin-fixed, paraffin-embedded mouse stomach stained with Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R) at 2 ug/ml. HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.
SDS-PAGE Analysis Purified Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R). Confirmation of Integrity and Purity of Antibody.
Western blot analysis of Jurkat cell lysate using Nucleolin Recombinant Rabbit Monoclonal Antibody (NCL/7014R).
Quick Database Links
Gene Symbol
NCL
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
NCL Antibody (orb1410331)
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