Recombinant Rat GRO-β/MIP-2/CXCL2 (rRtGRO-β/MIP-2/CXCL2)

SKU: orb1495002

Description

Rat GRO-beta/MIP-2/CXCL2 has been found to be expressed by cytokine stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, GRO-beta/MIP-2 is a member of the alpha (CXC) subfamily of chemokines. The protein sequence of rat GRO-beta/MIP-2 shares approximately 88% identity with murine MIP2. Characteristic of ELR containing CXC chemokines, GRO-beta/MIP-2 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo.

Images & Validation

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Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Key Properties

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Source	Escherichia coli.
Biological Activity	Fully biologically active when compared to standard. Determined by its ability to chemoattract total human neutrophils using a concentration range of 1.0-10.0 ng/ml, corresponding to a Specific Activity of ＞1 x 105 IU/mg.
Molecular Weight	7.9 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids.
Protein Sequence	VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN
Purification	>98% by SDS-PAGE and HPLC analyses.
Purity	>98% by SDS-PAGE and HPLC analyses.
Endotoxins	Less than 1EU/g of rRtMIP-2/CXCL2 as determined by LAL method.

Storage & Handling

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Storage	This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Form/Appearance	Lyophilized from a 0.2m filtered concentrated solution in PBS, pH 7.4.
Buffer/Preservatives	Lyophilized from a 0.2m filtered concentrated solution in PBS, pH 7.4.
Disclaimer	For research use only

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