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Recombinant Rat Vascular Endothelial Growth Factor 164, Yeast
SKU: orb1906113
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Description
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Key Properties
−| Source | Yeast |
|---|---|
| Expression System | Yeast |
| Biological Activity | Fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.75-3.75 ng/ml. |
| Molecular Weight | Theoretically as a disulfide-linked homodimeric protein, the product consists of two 165 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 25.7 kDa in SDS-PAGE under reducing conditions. |
| Protein Sequence | MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
| Purity | > 95 % by SDS-PAGE and 90% by SEC-HPLC analyses. |
| Endotoxins | Less than 0.1 EU/ug of rRtVEGF164, Yeast as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Disclaimer | For research use only |
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Recombinant Rat Vascular Endothelial Growth Factor 164, Yeast (orb1906113)
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