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S100B Antibody

SKU: orb1274274

Description

S100B Antibody

Research Area

Cell Biology, Signal Transduction

Images & Validation

Tested ApplicationsFC, IF, IHC-P, WB
ReactivityBovine, Human, Mouse, Rat
Application Notes
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLWestern blot: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.25-0.5 µg/mL for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)The optimal dilution of the recombinant S100B antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG, kappa
Clone No.S100B/1706R
ImmunogenFull length human recombinant S100B protein was used as the immunogen for this recombinant S100B antibody.
TargetS100B
PurificationProtein A affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration0.2 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

S100B Antibody

IHC testing of FFPE human melanoma with recombinant S100B antibody (clone S100B/1706R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

S100B Antibody (orb1274274)

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Available Sizes

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100 μg
$ 1,070.00
DispatchUsually dispatched within 5-10 working days
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