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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 7
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:2000, IHC-P-Leica - 1:500, FC - 1:10-50, IHC - 1:1000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Rabbit |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD44156 |
| Immunogen | This S100B antibody is generated from rabbits immunized with S100B recombinant protein. |
| Target | S100B {ECO:0000303|PubMed:6487634, ECO:0000312|HGNC:HGNC:10500} |
| Molecular Weight | 10713 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B
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S100B Antibody flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of paraffin-embedded Human brain section using Pink1. Diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded Human breast tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human melanoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Anti-S100B Antibody at 1:2000 dilution + Human brain whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 11 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of lysates from A431 cell line, mouse brain, rat brain tissue (from left to right), using S100B Antibody. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
All lanes: Anti-S100B Antibody at 1:2000 dilution. Lane 1: Human brain lysate. Lane 2: C2C12 whole cell lysate. Lane 3: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 11 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
Gene Symbol
S100B {ECO:0000303|PubMed:6487634, ECO:0000312|HGNC:HGNC:10500}
UniProt
RefSeq (Protein):NP_006263.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_006263.1 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
S100B Antibody (orb1930681)
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