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Recombinant SARS-Cov-2 Spike RBD protein (Y453F), His
SKU: orb1516702
Featured
Description
Research Area
SARS /COVID-19
Images & Validation
−Item 1 of 2
Key Properties
−| Expression System | E.coli |
|---|---|
| Target | Spike glycoprotein |
| Tag | His |
| Molecular Weight | 36 kDa |
| Protein Sequence | 319-541/1273 |
| Purification | AC |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxins | Not analyzed |
Storage & Handling
−| Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized or Liquid |
| Buffer/Preservatives | PBS (pH7.4). |
| Concentration | >0.5 mg/ml |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SARS-CoV-2 spike protein; 2019-nCOV Spike protein; Spike glycoprotein; Spike protein S1; Surface glycoprotein; Spike protein RBD; SPIKE_SARS2; 2019-nCOV Spike RBD (Y453F), Recombinant (His Tag); Recombinant SARS-Cov-2 Spike RBD protein (Y453F), His
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> 95% as determined by SDS-PAGE and Coomassie blue staining
30.1 KDa
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Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: orb1516578) at 2 µg/ml (100 ul/Well) can bind SARS-CoV-2 Spike RBD (Y453F), His Tag (Cat: orb1516702), the EC50 is 8.34 ng/ml.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
Quick Database Links
Gene Symbol
Spike glycoprotein
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Protocol Information
Recombinant SARS-Cov-2 Spike RBD protein (Y453F), His (orb1516702)
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