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WT1 Antibody

SKU: orb1274231

Description

WT1 Antibody

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin

Images & Validation

Tested ApplicationsFC, IF, IHC-P
ReactivityHuman, Rat
Application Notes
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant WT1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG, k
Clone No.WT1/1434R
ImmunogenA recombinant protein was used as the immunogen for the recombinant WT1 antibody.
TargetWT1
PurificationProtein A affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration0.2 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

WT1, GUD, WAGR, WIT-2, WT33, AWT1, NPHS4, EWS-WT1

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WT1 Antibody

IHC testing of FFPE human mesothelioma tissue with recombinant WT1 antibody (clone WT1/1434R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

WT1 Antibody

IHC testing of FFPE rat testis tissue with recombinant WT1 antibody (clone WT1/1434R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

WT1 Antibody

SDS-PAGE analysis of purified, BSA-free recombinant WT1 antibody (clone WT1/1434R) as confirmation of integrity and purity.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

WT1 Antibody (orb1274231)

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Available Sizes

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100 μg
$ 1,140.00
DispatchUsually dispatched within 5-10 working days
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