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WT1 Antibody

SKU: orb1274254

Description

WT1 Antibody

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin

Images & Validation

Tested ApplicationsIF, IHC-P
ReactivityHuman
Application Notes
Immunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Optimal dilution of the WT1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG, k
Clone No.RMWT1-1
ImmunogenA recombinant protein was used as the immunogen for the WT1 antibody.
TargetWT1
PurificationProtein A affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration0.2 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

WT1, GUD, WAGR, WIT-2, WT33, AWT1, NPHS4, EWS-WT1

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WT1 Antibody

Formalin-fixed, paraffin-embedded human kidney stained with WT1 antibody (clone RMWT1-1).

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol

WT1 Antibody (orb1274254)

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100 μg
$ 1,140.00
DispatchUsually dispatched within 5-10 working days
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