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WT1 Antibody (Center E361)
SKU: orb1937831
Description
Research Area
Cancer Biology, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 5
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, FC - 1:10-50, IHC-P - 1:100-500, IF - 1:10-50 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Porcine, Xenopus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 49188 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Quality Guarantee
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All lanes: Anti-WT1 Antibody (Center E361) at 1:1000 dilution. Lane 1: HT-1080 whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: MOLT-4 whole cell lysate. Lane 4: Mouse kidney lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 49 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-WT1 Antibody (Center E361) at 1:1000 dilution + Mouse testis lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 49 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of WT1 Antibody (Center E361) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Overlay histogram showing Hela cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
WT1 Antibody (Center E361) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of WT1 Antibody (Center E361) for immunohistochemistry. Clinical relevance has not been evaluated.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
WT1 Antibody (Center E361) (orb1937831)
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