RecombinantEndostatin,Human

SKU: orb1494618

Description

Endostatin is a cleaved product of the carboxyl-terminal domain of collagen XVIII. It functions as an anti-angiogenic cytokine that is expressed in various organs especially with high levels in liver, lung, and kidney. Endostatin inhibits angiogenesis by blocking the pro-angiogenic activities of VEGF and FGF-basic. Recombinant Endostatin expressed in yeast, whichcauses G1 arrest of endothelial cells. Endostatin treatment results in apoptosis of HUVE and HMVE cells. Recombinant human Endostatin is a 20.2 kDa protein consisting of 184 amino acid residues.

Images & Validation

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Application Notes	Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

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Source	P. pastoris
Biological Activity	Determined by Endothelial Cell Tube Formation. The cells treated with 10μg/ml Endostatin showed significant anti-angiogenesis effect.
Molecular Weight	~20.2 kDa, observed by reducing SDS-PAGE.
Protein Sequence	AH SHRDFQPVLH LVALNSPLSG GMRGIRGADF QCFQQARAVG LAGTFRAFLS SRLQDLYSIV RRADRAAVPI VNLKDELLFP SWEALFSGSE GPLKPGARIF SFDGKDVLRH PTWPQKSVWH GSDPNGRRLT ESYCETWRTE APSATGQASS LLGGRLLGQS AASCHHAYIV LCIENSFMTA SK
Purification	Greater than 97% by SDS-PAGE
Purity	Greater than 97% by SDS-PAGE
Endotoxins	Endotoxin level is less than 0.1 ng per μg (1EU/μg) of Endostatin.

Storage & Handling

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Storage	The lyophilized Endostatin is stable for a few weeks at room temperature, but best stored at -20°C. For the first use, reconstitute in water, aliquot and store at -20°C to avoid multiple freeze-thaws.
Form/Appearance	The sterile filtered solution was lyophilized with 17 mM citric-phosphate buffer, pH 6.2.
Buffer/Preservatives	The sterile filtered solution was lyophilized with 17 mM citric-phosphate buffer, pH 6.2.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Endostatin

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