RecombinantEnterokinase,LightChain,Porcine

SKU: orb1494616

Description

GenScript Enterokinase is a highly purified recombinant porcine enterokinase. The enzyme has been extensively purified and tested to ensure that there are no other contaminating proteases. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not cleave, however, if this lysine is followed by a proline. Enterokinase can remove fusion tags is located in the N-terminal section of proteins, useful for removing unwanted tags.

Images & Validation

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Application Notes	Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

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Source	P. pastoris
Biological Activity	One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25 mM Tris-HCl, pH 8.0.
Molecular Weight	Theoretical MW: 21,880 DaThe apparent MW on SDS-PAGE: about 40,000 Da
Protein Sequence	0

Storage & Handling

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Storage	Store at -20°C after delivery.
Form/Appearance	GenScript enterokinase, formulated using GenScript's proprietary technology, can be shipped at room temperature. It will remain stable at 37°C for one week without losing any activity.
Buffer/Preservatives	GenScript enterokinase, formulated using GenScript's proprietary technology, can be shipped at room temperature. It will remain stable at 37°C for one week without losing any activity.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Enteropeptidase, ENTK, PRSS7

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