RecombinantFGF-18,Mouse

SKU: orb1494883

Description

Fibroblast Growth Factor 18 (FGF-18) is a pleiotropic cytokine belonging to the heparin-binding FGF family, which has 23 different members. Structurally, FGF-18 is closely related to FGF-8 and FGF-17. Like other FGFs, FGF-18 can bind to different FGF receptors in vivo. FGF-18 is expressed in various tissues and has multiple functions: during long bone growth, FGF-18 is expressed in perichondrium and developing joints, and regulates bone formation by inhibiting chondrocyte proliferation and differentiation; FGF-18 knock-out mice survive embryonic development, but exhibit skeletal abnormalities and die in the early neonatal period. FGF-18 also induces ectopic cartilage formation in the lung, and alters the morphology of the pulmonary mesenchyma. Recombinant mouse Fibroblast Growth Factor 18 (rmFGF-18) produced in E.coli is a single non-glycosylated polypeptide chain containing of 172 amino acids. A fully biologically active molecule, rmFGF-18 has a molecular mass of 20.1 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/mL.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 2× 10ˆ3 units/mg.
Molecular Weight	20.1 kDa, observed by non-reducing SDS-PAGE.
Protein Sequence	EEN VDFRIHVENQ TRARDDVSRK QLRLYQLYSR TSGKHIQVLG RRISARGEDG DKYAQLLVET DTFGSQVRIK GKETEFYLCM NRKGKLVGKP DGTSKECVFI EKVLENNYTA LMSAKYSGWY VGFTKKGRPR KGPKTRENQQ DVHFMKRYPK GQAELQKPFK YTTVTKRSR
Purification	> 95% by SDS-PAGE and HPLC analyses.
Purity	> 95% by SDS-PAGE and HPLC analyses.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant mouse Fibroblast Growth Factor 18 (rmFGF-18) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmFGF-18 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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FGF18, Fibroblast Growth Factor-18, zFGF5, FGFI

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