RecombinantFGF-21,Mouse

SKU: orb1494880

Description

Fibroblast growth factor-21 (FGF21) belongs to the large FGF family which has at least 23 members. Along with FGF-19/15 and FGF-23, FGF-21 is categorized as a member of the atypical FGF subfamily, as it must be complexed to the Klotho co-receptor in order to bind to the FGF receptors and activate the downstream signaling pathway. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Recombinant Mouse Fibroblast Growth Factor-21(FGF-21) produced in E.coli is a single non-glycosylated polypeptide chain containing 182 amino acids. A fully biologically active molecule, rmFGF-21 has a molecular mass of 19.9 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 2 × 10ˆ3 units/mg.
Molecular Weight	19.9 kDa, observed by reducing SDS-PAGE.
Protein Sequence	AYP IPDSSPLLQF GGQVRQRYLY TDDDQDTEAH LEIREDGTVV GAAHRSPESL LELKALKPGV IQILGVKASR FLCQQPDGAL YGSPHFDPEA CSFRELLLED GYNVYQSEAH GLPLRLPQKD SPNQDATSWG PVRFLPMPGL LHEPQDQAGF LPPEPPDVGS SDPLSMVEPL QGRSPSYAS
Purification	> 97% as analyzed by SDS-PAGE& HPLC.
Purity	> 97% as analyzed by SDS-PAGE& HPLC.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant Mouse Fibroblast Growth Factor-21(FGF-21), remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Mouse FGF-21 should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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FGF21, fibroblast growth factor-21, FGFL

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