RecombinantFGF-4,Human

SKU: orb1494879

Description

Fibroblast Growth Factor-4 (FGF-4) also known as K-FGF is a heparin-binding growth factor of the FGF family.It was identified by its oncogenic transforming activity. FGF-4 and FGF-3 are located closely on chromosome 11. FGF-4 and its receptors (FGF R1c, 2c, 3c and 4) play an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF-4 is required for normal limb and cardiac valve development during embryogenesis.Recombinant human Fibroblast Growth Factor-4 (rhFGF-4) produced in E.coli is a single non-glycosylated polypeptide chain containing 177 amino acids. A fully biologically active molecule obtained by proprietary chromatographic techniques at GenScript, rhFGF-4 has a molecular mass of 19.4kDa as analyzed by reducing SDS-PAGE.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED502.0× 10ˆ6 units/mg.
Molecular Weight	19.4kDa, observed by reducing SDS-PAGE.
Protein Sequence	MAPTAPNGT LEAELERRWE SLVALSLARL PVAAQPKEAA VQSGAGDYLL GIKRLRRLYC NVGIGFHLQA LPDGRIGGAH ADTRDSLLEL SPVERGVVSI FGVASRFFVA MSSKGKLYGS PFFTDECTFK EILLPNNYNA YESYKYPGMF IALSKNGKTK KGNRVSPTMK VTHFLPRL
Purification	> 95% by SDS-PAGE and HPLC analyses.
Purity	> 95% by SDS-PAGE and HPLC analyses.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant human Fibroblast Growth Factor-4 (rhFGF-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against 50mM HEPES, 750mM NaCl, pH 7.5.
Buffer/Preservatives	Lyophilized after extensive dialysis against 50mM HEPES, 750mM NaCl, pH 7.5.
Disclaimer	For research use only

Alternative Names

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FGF4; HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF.

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