RecombinantFGF-8c,Mouse

SKU: orb1494875

Description

Fibroblast Growth Factor 8c (FGF-8c) is a cytokine belonging to the heparin-binding FGF family, which has at least 23 members. In different species, e.g. human and mouse, FGF-8 has 8 different isoforms, from FGF-8a to FGF-8h. Different FGF-8 isoforms have different affinities to the receptors, thus conduct different signaling cascade pathways. FGF-8 has very widespread expression pattern during embryonic development, and is an organizer and inducer for gastrulation, somitogenesis, morphogenesis, and limb induction. However, FGF-8 is also a potential oncogene: in normal adult cells, FGF-8 has very low expression; on the other hand, FGF-8 is highly expressed in cancer cells of breast, prostate, and ovarian tumors. FGF-8 promotes tumor angiogenesis by increasing neovascularization, and induces osteoblastic differentiation.Recombinant mouse Fibroblast Growth Factor 8c (rmFGF-8c) produced in E.coli is a single non-glycosylated polypeptide chain containing 247 amino acids. A fully biologically active molecule, rmFGF-8c has a molecular mass of 28.2 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/mL.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 6.7× 10ˆ3 units/mg.
Purification	> 95% by SDS-PAGE and HPLC analyses.
Endotoxins	< 0.2 EU/μg, determined by LAL method.
MW	28.2 kDa, observed by reducing SDS-PAGE.
Purity	> 95% by SDS-PAGE and HPLC analyses.
Protein Sequence	MQVRSAAQK RGPGAGNPAD TLGQGHEDRP FGQRSRAGKN FTNPAPNYPE EGSKEQRDSV LPKVTQRHVR EQSLVTDQLS RRLIRTYQLY SRTSGKHVQV LANKRINAMA EDGDPFAKLI VETDTFGSRV RVRGAETGLY ICMNKKGKLI AKSNGKGKDC VFTEIVLENN YTALQNAKYE GWYMAFTRKG RPRKGSKTRQ HQREVHFMKR LPRGHHTTEQ SLRFEFLNYP PFTRSLRGSQ RTWAPEPR

Storage & Handling

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Storage	Lyophilized recombinant mouse Fibroblast Growth Factor 8c (rmFGF-8c) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmFGF-8c should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Fibroblast growth factor 8, FGF-8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8, Fgf8

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