RecombinantFGF-basic(145aa),Human

SKU: orb1494868

Description

Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.Recombinant Human FGF-basic (145a.a.)produced in E.coli is a single non-glycosylated polypeptide chain containing 145 amino acids. A fully biologically active molecule, rhFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 4 × 10ˆ6 units/mg
Molecular Weight	16.4 kDa, observed by reducing SDS-PAGE.
Protein Sequence	ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEER G VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKR T GQYKLGSKTG PGQKAILFLP MSAKS
Purification	> 95% as analyzed by SDS-PAGE.
Purity	> 95% as analyzed by SDS-PAGE.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant Human FGF-basic (145a.a.) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human FGF-basic (145a.a.) should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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Fibroblast Growth Factor-basic, FGF-2, FGF2, HBGF-2, Prostatropin

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