RecombinantGRO-β/CINC-3/CXCL2,Rat

SKU: orb1494851

Description

Chemokine (C-X-C motif) ligand 2(CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 shares 90% amino acid sequence with CXCL1/GROα. The GRO proteins are chemotactic for neutrophils and basophils and can activate them through their CXCR1 or CXCR2 receptors.Recombinant Rat GRO-β/CINC-3/CXCL2 produced in E.coli is a single non-glycosylated polypeptide chain containing 69 amino acids. A fully biologically active molecule, rrGRO-β has a molecular mass of 7.6kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Images & Validation

−

Application Notes	Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

−

Source	Escherichia coli.
Biological Activity	The EC50 value of Rat GRO-β/CINC-3/CXCL2 on Caˆ2+ mobilization assay in CHO-K1/Gα15/rCXCR2 cells (human Gα15 and rat CXCR2 stably expressed in CHO-K1 cells) is less than 10 ng/ml.
Molecular Weight	7.6 kDa, observed by reducing SDS-PAGE.
Protein Sequence	SE LRCQCLTTLP RVDFKNIQSL TVTPPGPHCA QTEVIATLKD GHEVCLNPEA PLVQRIVQKI LNKGKAN
Purification	> 95% as analyzed by SDS-PAGE.
Purity	> 95% as analyzed by SDS-PAGE.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

−

Storage	Lyophilized recombinant Rat GRO-β/CINC-3°CXCL2 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Rat GRO-β/CINC-3°CXCL2 should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

−

Growth Regulated Protein/Melanoma Growth Stimulatory Activity (MGSAβ), CXCL2, MIP-2, GRO2,CINC-3

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols