RecombinantGRO-β/MIP-2/CXCL2,Rat

SKU: orb1494850

Description

Rat CXCL2 also named CINC-3, is belonging to the CXC chemokine family. It is encoded by the gene CXCL2. CXCL2 shares 90% amino acid sequence with CXCL1/GROα. CINC-3 is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. This chemokine is expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. The functional receptor for CXCL2 has been identified as CXCR2. CXCL2 is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. Similar to other GRO proteins, CXCL2 is potent neutrophil attractants and activators. In addition, it is also active toward basophils. The amino acid sequence of rat CXCL2 is 88% identical to murine CXCL2.

Images & Validation

−

Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in appropriate buffered solutions.

Key Properties

−

Source	Escherichia coli.
Biological Activity	Fully biologically active when compared to standard. Determined by its ability to chemoattract total human neutrophils using a concentration range of 1.0-10.0 ng/ml.
Molecular Weight	Approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids.
Protein Sequence	VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN
Purification	>98% by SDS-PAGE and HPLC analyses.
Purity	>98% by SDS-PAGE and HPLC analyses.
Endotoxins	Less than 1 EU/μg of rRtMIP-2/CXCL2 as determined by LAL method.

Storage & Handling

−

Storage	This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Form/Appearance	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Buffer/Preservatives	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Disclaimer	For research use only

Alternative Names

−

Growth Regulated Protein/Melanoma Growth Stimulatory Activity (MGSAβ), CXCL2, MIP-2, GRO2,CINC-3

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols