RecombinantIFN-α2b,Human

SKU: orb1494844

Description

Interferon-Alpha 2b (IFN-Alpha 2b) produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a,b,c) signaling through IFNAR2. IFN-alpha 2b shares 99.4% aa sequence identity with both IFN-alpha 2a and 2c. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.Recombinant human Interferon-Alpha 2b (rhIFN-Alpha 2b) produced in E.coli is a single non-glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rhIFN-Alpha 2b has a molecular mass of 19.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 2.0× 10ˆ7 units/mg.
Purification	> 95% by SDS-PAGE and HPLC analyses.
Endotoxins	< 0.2 EU/μg, determined by LAL method.
MW	19.2kDa, observed by reducing SDS-PAGE.
Purity	> 95% by SDS-PAGE and HPLC analyses.
Protein Sequence	CDL PQTHSLGSRR TLMLLAQMRR ISLFSCLKDR HDFGFPQEEF GNQFQKAETI PVLHEMIQQI FNLFSTKDSS AAWDETLLDK FYTELYQQLN DLEACVIQGV GVTETPLMKE DSILAVRKYF QRITLYLKEK KYSPCAWEVV RAEIMRSFSL STNLQESLRS KE

Storage & Handling

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Storage	Lyophilized recombinant human Interferon-Alpha 2b (rhIFN-Alpha 2b) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIFN-Alpha 2b should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Interferon alpha 2b, IFNA, INFA2

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