RecombinantIL-10,Rat

SKU: orb1494834

Description

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine produced by a variety of cell lines including T-cells, macrophages and mast cells. IL-10 is classified as a class-2 cytokine, a set of cytokines including IL-19, IL-20, IL-22, IL-24, and IL-26. IL-10 can inhibit the synthesis of pro-inflammatory cytokines such as IFN-gamma, IL-2, IL-3, TNF and GM-CSF. It also stimulates Th2 responses, but suppresses the antigen-presentation capacity of antigen presenting cells.Recombinant rat Interleukin-10 (IL-10)produced in E.coli is a single non-glycosylated polypeptide chain containing 161 amino acids. A fully biologically active molecule, recombinant rat Interleukin-10 (IL-10) has a molecular mass of 18.7kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 2.0× 10ˆ3 units/mg.
Molecular Weight	18.7 kDa, observed by reducing SDS-PAGE.
Protein Sequence	MSKGHSIKG DNNCTHFPVS QTHMLRELRA AFSQVKTFFQ KKDQLDNIVL TDSLLQDFKG YLGCQALSEM IKFYLVEVMP QAENHGPEIK EHLNSLGEKL KTLWIQLRRC HRFLPCENKS KAVEQVKNDF NKLQDKGVYK AMNEFDIFIN CIEAYVTLKM KN
Purification	> 95% by SDS-PAGE and HPLC analyses.
Purity	> 95% by SDS-PAGE and HPLC analyses.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant rat Interleukin-10 (IL-10) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, IL-10 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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Interleukin-10, IL10

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