RecombinantIL-15,His,Human

SKU: orb1494833

Description

Interleukin-15 (IL-15) is a cytokine with structural similarity to IL-2. Like IL-2, IL-15 binds to and signals through a complex composed of IL-2/IL-15 receptor beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (amongother cells) following infection by virus. This cytokine induces cell proliferation of natural killer cells, which are cells of the innate immune system whose principal role is to kill virally infected cells. IL-15 can stimulate the proliferation of T-lymphocytes. Stimulation by IL-15 occurs following its interaction withIL-15Rα. This interaction may enhance IL-15’s interaction with IL15Rβγc. Recombinant Human IL-15 (His-tag) produced in E.coli is a single non-glycosylated polypeptide chain containing 121 amino acids. A fully biologically active molecule, rhIL-15 (His-tag) has a molecular mass of 13.6 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 2 x 10ˆ6 units/mg.
Molecular Weight	13.6 kDa, observed by reducing SDS-PAGE.
Protein Sequence	MHHHHHHNWV NVISDLKKIE DLIQSMHIDA TLYTESDVHP SCKVTAMKCF LLELQVISLE SGDASIHDTV ENLIILANNS LSSNGNVTES GCKECEELEE KNIKEFLQSF VHIVQMFINT S
Purification	> 95% as analyzed by SDS-PAGE.
Purity	> 95% as analyzed by SDS-PAGE.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant Human IL-15(His-tag) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human IL-15(His-tag) should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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Interleukin-15, IL15

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