RecombinantIL-18,Rat

SKU: orb1494831

Description

Interleukin-18 (IL18, also known as interferon-gamma inducing factor or IGIF) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. Its biological activity is pleiotropic and it has been shown to induce interferon-gamma production in KG-1 cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.Recombinant rat Interleukin-18 (rrIL-18) produced in E.coli is a single non-glycosylated polypeptide chain containing 159 amino acids. A fully biologically active molecule, rrIL-18 has a molecular mass of 18.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 500 units/mg.
Molecular Weight	18.4 kDa, observed by reducing SDS-PAGE.
Protein Sequence	MHFGRLHCT TAVIRSINDQ VLFVDKRNPP VFEDMPDIDR TANESQTRLI IYMYKDSEVR GLAVTLSVKD GRMSTLSCKN KIISFEEMNP PENIDDIKSD LIFFQKRVPG HNKMEFESSL YEGHFLACQK EDDAFKLVLK RKDENGDKSV MFTLTNLHQS
Purification	> 95% by SDS-PAGE analyses.
Purity	> 95% by SDS-PAGE analyses.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant ratInterleukin-18 (rrIL-18) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrIL-18 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against 50mM Tris, 50mM NaCl, pH 8.0.
Buffer/Preservatives	Lyophilized after extensive dialysis against 50mM Tris, 50mM NaCl, pH 8.0.
Disclaimer	For research use only

Alternative Names

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Interleukin-18, IL18

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