RecombinantIP-10/CXCL10,Human

SKU: orb1494811

Description

IP-10/CXCL10 also known as CXCL10, is originally identified as an IFN-γ-inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that IP-10 mRNA is also induced by LPS, IL-1β, TNF-α, IL-12 and viruses. Additional cell types that have been shown to express IP-10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. IP-10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human IP-10, Crg-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human IP-10.Recombinant human IP-10/CXCL10 (rhIP-10) produced in E. coli is a single non-glycosylated polypeptide chain containing 78 amino acids. A fully biologically active molecule, rhIP-10 has a molecular mass of 8.6kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 5.0× 10ˆ3 IU/mg.
Molecular Weight	8.6 kDa, observed by reducing SDS-PAGE.
Protein Sequence	MVPLSRTVR CTCISISNQP VNPRSLEKLE IIPASQFCPR VEIIATMKKK GEKRCLNPES KAIKNLLKAV SKEMSKRSP
Purification	> 95% by SDS-PAGE and HPLC analyses.
Purity	> 95% by SDS-PAGE and HPLC analyses.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant human IP-10°CXCL10 (rhIP-10) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIP-10 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against 50mM Tris, pH 8.0.
Buffer/Preservatives	Lyophilized after extensive dialysis against 50mM Tris, pH 8.0.
Disclaimer	For research use only

Alternative Names

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Small inducible cytokine B10, CXCL10, 10 kDa interferon-gamma-induced protein, Gamma-IP10, IP-10, chemokine (C-X-C motif) ligand 10, C7, IFI10, INP10, crg-2, mob-1, SCYB10, gIP-10

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