RecombinantPDGF-BB,Human(P.pastoris-expressed)

SKU: orb1494612

Description

Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle, connective tissue, bone and cartilage cells, and some blood cells. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer.Recombinant human Platelet-Derived Growth Factor-BB (rhPDGF-BB) produced in Pichia pastoris is a glycosylated polypeptide. rhPDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids) and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/ml.

Key Properties

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Source	P. pastoris
Biological Activity	ED50 3.3 x 10ˆ5 units/mg.
Molecular Weight	24.3kDa, observed by non-reducing SDS-PAGE.
Protein Sequence	SL GSLTIAEPAM IAECKTRTEV FEISRRLIDR TNANFLVWPP CVEVQRCSGC CNNRNVQCRP TQVQLRPVQV RKIEIVRKKP IFKKATVTLE DHLACKCETV AAARPVT
Purification	> 97% by SDS-PAGE and HPLC analyses.
Purity	> 97% by SDS-PAGE and HPLC analyses.
Endotoxins	< 1.0 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant human Platelet-Derived Growth Factor-BB (rhPDGF-BB) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhPDGF-BB should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against 10mM acetic acid.
Buffer/Preservatives	Lyophilized after extensive dialysis against 10mM acetic acid.
Disclaimer	For research use only

Alternative Names

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PDGF

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