RecombinantPDGF-BB,Rat

SKU: orb1494777

Description

Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo, PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, human skeletal development, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rrPDGF-BB has a molecular mass of 24.6 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in 20 mM acetic acid at 100 μg/mL.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 5 × 10ˆ5 units/mg.
Molecular Weight	24.6 kDa, observed by non-reducing SDS-PAGE.
Protein Sequence	MSLGSLAAA EPAVIAECKT RTEVFQISRN LIDRTNANFL VWPPCVEVQR CSGCCNNRNV QCRASQVQMR PVQVRKIEIV RKKPVFKKAT VTLEDHLACK CETVVTPRPV T
Purification	> 95% by SDS-PAGE analysis.
Purity	> 95% by SDS-PAGE analysis.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant rat Platelet-Derived Growth Factor-BB (rrPDGF-BB) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrPDGF-BB remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against 20 mM acetic acid.
Buffer/Preservatives	Lyophilized after extensive dialysis against 20 mM acetic acid.
Disclaimer	For research use only

Alternative Names

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PDGF

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