RecombinantTEVProtease,His

SKU: orb1494770

Description

Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C; also it can be used at temperature as low as 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant Tobacco Etch Virus Protease (rTEV) contains 231 amino acids with N-terminal His tagged. A fully biologically active molecule, rTEV has a molecular mass of 28.4 kDa and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Key Properties

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Source	Escherichia coli.
Biological Activity	6 IU/μl. Unit Definition: One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.
Molecular Weight	28.4 kDa, observed by reducing SDS-PAGE.
Protein Sequence	0
Purification	> 95% by SDS-PAGE analyses.
Purity	> 95% by SDS-PAGE analyses.

Storage & Handling

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Storage	Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20°C from date of receipt. Please avoid freeze-thaw cycles.
Form/Appearance	Sterile liquid solution contains 50mM Tris, 5mM DTT, 50% glycerol, pH 7.5.
Buffer/Preservatives	Sterile liquid solution contains 50mM Tris, 5mM DTT, 50% glycerol, pH 7.5.
Disclaimer	For research use only

Alternative Names

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rTEV, TEV, P1 protease

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