Bevacizumab Human Monoclonal Antibody

SKU: orb2977969

Description

Bevacizumabis a research-grade recombinant antibody targeting Vascular endothelial growth factor A. Produced in mammalian cells with native-like glycosylation. Highlights Research Grade: For PK/PD studies, assay development, and ADA research. Native Glycosylation: Mammalian expression ensures native-like patterns.

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FA, FACS, In vivo
Reactivity	Human

Key Properties

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Antibody Type	Biosimilar Antibody
Host	Human Humanized
Clonality	Monoclonal
Isotype	IgG1-kappa
Source/Expression System	Mammalian Cells
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin	Please contact with the lab for this information.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS, pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Detects Human VEGF165 in indirect ELISAs.

Flow-cytometry using anti-human VEGFA antibody. U937 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA monoclonal antibody (Catalog orb2977969, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using FITC anti-human VEGFA antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGFA monoclonal antibody (Catalog: orb2977969, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Research Grade Bevacizumab.

Quick Database Links

UniProt

P15692

UniProt Details

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No UniProt data available

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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