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TNFSF15/TL1A & TNFa/TNF-alpha Human Monoclonal Antibody
SKU: orb2976475
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 8
| Tested Applications | ELISA, FA, FACS, In vivo |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Biosimilar Antibody |
|---|---|
| Host | Human |
| Clonality | Monoclonal |
| Isotype | IgG1-kappa |
| Clone No. | BCOI188 |
| Source/Expression System | Mammalian Cells |
| Purity | >95% purity as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin | Please contact with the lab for this information. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M PBS, pH 7.4. |
| Concentration | 2.06 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TNFSF15, Vascular endothelial cell growth inhibitor, VEGI, TL1, TNF ligand-related molecule 1, Tumor necrosis factor ligand superfamily member 15, TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
Detects recombinant human TNFa (Cat No.: orb2964282) in indirect ELISA.
Detects recombinant human TNFa (Cat No.: orb2969650) in indirect ELISA.
Detects Recombinant Human TNFSF15/TL1A Protein, C-His (Cat No.: orb2964282) in indirect ELISA.
Flow-cytometry APC using anti-human TNFa antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human TNFa monoclonal antibody (Catalog: orb2976475) at a concentration of 5 µg/mL for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry FITC using anti-human TNFa antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human TNFa monoclonal antibody (Catalog: orb2976475) at a concentration of 5 µg/mL for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human TNFa antibody. 293T Transtected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog: orb2976475, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Research Grade Anti-Human TNFSF15/TL1A & TNFa/TNF-alpha Antibody.
Western blot analysis was performed using anti-TNFSF15/TL1A monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human TNFSF15/TL1A, Lane 2: negative control.
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Protocol Information
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
TNFSF15/TL1A & TNFa/TNF-alpha Human Monoclonal Antibody (orb2976475)
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