Rhesus macaque IL-12B&IL-12A Heterodimer Protein, His Tag&His Tag (MALS verified)

SKU: orb3158161

Description

Interleukin 12 (IL12) is also known as p70, and is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.IL12 is a heterodimeric cytokine, containing IL-12A (p35) and IL-12B (p40). IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T cells and NK cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.

Images & Validation

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Application Notes

Rhesus macaque IL-12B&IL-12A Heterodimer Protein, His Tag&His Tag, produced by co-expression of IL-12B and IL-12A, has a calculated MW of 36.7 kDa (IL-12B) and 24.4 kDa (IL-12A). Subunit IL-12B is fused with a polyhistidine tag at the C-terminus, and subunit IL-12A contains a polyhistidine tag at the C-terminus. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Target	IL-12B & IL-12A
Reactivity	Monkey
Tag	C-10×His \| C-10×His
Molecular Weight	36.7 kDa & 24.4 kDa
Expression Region	Ile 23 - Ser 328 & Arg 23 - Ser 219
Purity	90%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

IL-12B & IL-12A

RefSeq:P48095, P48091

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	P48095, P48091

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