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RIPK3 Antibody (Center)
SKU: orb1929500
Description
Research Area
Cell Biology, Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 56887 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIP3 (RIPK3) antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of RIP3 (RIPK3) Antibody (Center) in Hela cell line lysates (35 ug/lane). RIPK3 (arrow) was detected using the purified Pab.
Western blot analysis of RIP3 (RIPK3) Antibody (Center) in mouse cerebellum tissue lysates (35 ug/lane). RIPK3 (arrow) was detected using the purified Pab.
Western blot analysis of lysates from HT-29, K562 cell line (from left to right), using RIPK3 Antibody Center. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
RIPK3 Antibody (Center) (orb1929500)
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