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RK33

SKU: orb1300301

Description

RK33 is a first-in-class small molecule DDX3 inhibitor. It induces G1 cell cycle arrest and apoptosis while promoting radiation sensitization in DDX3-overexpressing cells. This compound is a valuable tool for oncology research, particularly in studying DDX3's role in cancer cell proliferation and radiosensitization in vitro.

Research Area

Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Molecular Biology, Neuroscience

Images & Validation

Key Properties

CAS Number1070773-09-9
MW428.44
Purity99.72%
FormulaC23H20N6O3
SMILESCOc1ccc(Cn2cnc3c4nc(=O)n(Cc5ccc(OC)cc5)c4ncnc23)cc1
TargetSARS-CoV,COX,DNA/RNA Synthesis
SolubilityH2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:25 mg/mL (58.35 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

In Vivo
RK-33 in combination with radiation induces tumor regression in multiple mouse models of lung cancer. RK-33, at the dose used, is non-toxic in SCID mice. RK-33-treated mice do not exhibit any discernable morphological changes.
In Vitro
RK-33 binds specifically to DDX3, but not to the closely related proteins DDX5 and DDX17. RK-33 inhibits cancer growth and radiosensitizes lung cancer cells in a DDX3-dependent manner. RK-33 has no effect on either mitochondrial respiration or ATP generation. RK-33 curbs proliferation and induces apoptosis in a DDX3-dependent fashion. Wnt signaling is mediated by DDX3 and inhibited by RK-33. RK-33 impairs radiation-induced DNA damage repair by inhibiting NHEJ activity.
Cell Research
Healthy, 60-70% confluent MDA-MB-231 cells are transduced with shDDX3 lentivirus particles. Knockdown of DDX3 expression is confirmed both by qRT-PCR and immunoblotting. MDA-MB-231 cells are treated with RK-33 (7.5 μM) for 12 h and harvested for RNA. Microarray experiments are performed.(Only for Reference)

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

DDX3, RK 33, RK33, RK-33

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  • RK-33 [orb1223437]

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    1070773-09-9

    428.44

    C23H20N6O3

    200 mg, 100 mg, 10 mg, 25 mg, 5 mg, 50 mg, 2 mg, 1 g, 500 mg
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Key Properties

No computed properties available.

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RK33 (orb1300301)

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% DMSO +
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% Tween 80 +
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Available Sizes

Select a size below

2 mg
$ 70.00
5 mg
$ 90.00
1 ml x 10 mM (in DMSO)
$ 100.00
10 mg
$ 120.00
25 mg
$ 190.00
50 mg
$ 270.00
100 mg
$ 420.00
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