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Rottlerin

SKU: orb1307775

Description

Rottlerin (NSC-56346), a natural compound from Mallotus philippinensis, is a selective PKCδ inhibitor (IC50 3-6 μM) that induces apoptosis through caspase-3 activation. It is widely used in biochemical research to study PKC isoform-specific signaling and programmed cell death in various cancer and cellular models.

Research Area

Cell Biology, Epigenetics & Chromatin, Infectious Disease & Virology, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

Key Properties

CAS Number82-08-6
MW516.54
Purity99.44%
FormulaC30H28O8
SMILESCC(=O)c1c(O)c(C)c(O)c(Cc2c(O)c3C=CC(C)(C)Oc3c(C(=O)\C=C\c3ccccc3)c2O)c1O
TargetAutophagy,PKA,HIV Protease,PKC,Apoptosis,CaMK
SolubilityDMSO:6.02 mg/mL (11.65 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (1.94 mM)

Bioactivity

Target IC50
PKCγ:40 μM(baculovirus-infected Sf9 insect cells)|PKA:78 μM|PKCα:30 μM(baculovirus-infected Sf9 insect cells)|PKCε:100 μM(baculovirus-infected Sf9 insect cells)|PKCβ:42 μM(baculovirus-infected Sf9 insect cells)|CK2:30 μM(holoenzyme expressed in E.coli)|PKCη:82 μM(baculovirus-infected Sf9 insect cells)|CaMK II:5.3 μM (EF-2 Kinase activity in cytosol of murine pancreas)|PKCζ:100 μM(baculovirus-infected Sf9 insect cells)|PKCδ:3 μM(Porcine spleen)
In Vivo
Rottlerin inhibits tumor cell proliferation and causes apoptosis through activation of caspase-3 and cleavage of the poly polymerase. Rottlerin (20 mg/kg, gavage 5 days per week, once daily, for 6 weeks) inhibits AsPC-1 pancreatic tumor growth in Balb C nude mice with no toxicity .
In Vitro
Rottlerin (20 μM) shows cell proliferation in HMVEC. Rottlerin (20 μM, 2/6/24 hours) dramatically reduces the cyclin D-1 mRNA levels in a time-dependent manner in primary HMVEC .

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CKII, CaM kinase III, Autophagy, Apoptosis, Mallotoxin, HIV, HIVProtease, HIV Protease, Human immunodeficiency virus, inhibit, NSC56346, NSC-56346, NSC 56346, NSC 94525, Inhibitor, NSC94525, NSC-94525, PKA, PKC, PKCα, PKCβ, PKCγ, PKCδ, PKCε, PKCζ, PKCη, Protein kinase C, Rottlerin
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Key Properties

No computed properties available.

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Protocol Information

Rottlerin (orb1307775)

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10 mg
$ 170.00
50 mg
$ 350.00
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