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RPA p32 (phospho Ser33) rabbit pAb Antibody
SKU: orb764314
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Description
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| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RFA2 around the phosphorylation site of Ser33. AA range:1-50 |
| Molecular Weight | 32 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-RPA2 antibody, anti-REPA2 antibody, anti-RPA32 antibody, anti-RPA34 antibody, anti-Replication protein A 32 kDa subunit antibody, anti-RP-A p32 antibody, anti-Replication factor A protein 2 antibody, anti-RF-A protein 2 antibody, anti-Replication protein A 34 kDa subunit antibody, anti-RP-A p34 antibody
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Western Blot analysis of various cells using Phospho-RPA p32 (S33) Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Western Blot analysis of MCF-7 cells using Phospho-RPA p32 (S33) Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Western blot analysis of lysates from NIH/3T3 cells treated with Adriamycin 0.5ug/ml 24h, using RFA2 (Phospho-Ser33) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
RPA p32 (phospho Ser33) rabbit pAb Antibody (orb764314)
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