RPS28 Rabbit Polyclonal Antibody

SKU: orb326551

Description

Rabbit polyclonal antibody to RPS28

Research Area

Cell Biology

Images & Validation

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Item 1 of 4

Tested Applications	IHC, WB
Reactivity	Human
Predicted Reactivity	Bovine, Equine, Goat, Guinea pig, Mouse, Rabbit, Rat, Yeast, Zebrafish

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the N-terminal region of Human RPS28
Target	RPS28
Protein Sequence	Synthetic peptide located within the following region: RTGSQGQCTQVRVEFMDDTSRSIIRNVKGPVREGDVLTLLESEREARRLR
Molecular Weight	8kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti S28 antibody

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Positive control (+): THP-1 (N30), Negative control (-): A549 (N03), Antibody concentration: 3 ug/mL.

Rabbit Anti-RPS28 Antibody, Catalog Number: orb326551, Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver, Observed Staining: Cytoplasm in Kupffer cells only, strong signal, very low tissue distribution, Primary Antibody Concentration: 1:100, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5 – 2.0 sec, Protocol located in Reviews and Data.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-RPS28 Antibody, Titration: 1.0 ug/mL, Positive Control: MDA-MB-435S Whole Cell, RPS28 is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB435 cells.

Quick Database Links

Gene Symbol

RPS28

UniProt

P62857

RefSeq (Protein):NP_001022

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001022

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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