RUNX2 Recombinant Rabbit Monoclonal Antibody

SKU: orb1499383

Description

Research Area

DNA and RNA Research, Osteogenesis, Transcription Factors

Images & Validation

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Item 1 of 1

Tested Applications	FC, ICC, WB
Dilution Range	WB=1:500-2000, Flow-Cyt=1:50-100, ICC/IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	8H9
Immunogen	A synthesized peptide derived from human RUNX2 (300-450/521aa)
Target	RUNX2
Molecular Weight	57 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Runx-2; RUNX2_HUMAN; Runt-related Transcription Factor 2; CBF alpha 1; CBF-alpha-1; PEBP2-alpha A; CBFA1; CCD; CCD1; Cleidocranial dysplasia 1; Core binding factor; Core binding factor runt domain alpha subunit 1; Core binding factor subunit alpha 1; MGC120023; Oncogene AML 3; OSF 2; OSF2; OSF-2; Osteoblast specific transcription factor 2; OTTHUMP00000016533; PEA2 alpha A; PEA2aA; PEBP2 alpha A; PEBP2A1; PEBP2A2; PEBP2aA1; Polyomavirus enhancer binding protein 2 alpha A subunit; Runt domain; Runt related transcription factor 2; SL3 3 enhancer factor 1 alpha A subunit; SL3/AKV core binding factor alpha A subunit; AML3; CLCD.

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RUNX2 Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Human MDA-MB-231 cell lysates, Lane 2: Human U251 cell lysates, Primary: Anti-RUNX2 (orb1499383) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kDa, Observed band size: 55 kDa.

Quick Database Links

Gene Symbol

RUNX2

UniProt

Q13950

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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ICC

Immunocytochemistry

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