RV01

SKU: orb1708629

Description

RV01 is a quinoline-substituted resveratrol analog with potent antioxidant and anti-inflammatory properties. It functions by scavenging hydroxyl radicals, suppressing NADPH oxidase, and inhibiting the LPS-induced TLR4/MAPK/NF-κB pathway, thereby reducing pro-inflammatory cytokine production. This mechanism supports its research applications in studying neuroinflammation and oxidative stress-related pathways in cellular and animal models.

Research Area

Immunology & Inflammation, Metabolism Research, Signal Transduction

Images & Validation

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Key Properties

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CAS Number	1016897-10-1
MW	263.29
Purity	99.93% (May vary between batches)
Formula	C₁₇H₁₃NO₂
SMILES	Oc1cc(O)cc(\C=C\c2ccnc3ccccc23)c1
Target	TLR,Dehydrogenase,MAPK,NF-κB
Solubility	DMSO:45 mg/mL (170.91 mM)

Bioactivity

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In Vitro

The anti-neuroinflammatory effect of a novel quinolyl-substituted analogue of resveratrol (RV01) on lipopolysaccharide (LPS)-induced microglial activation is investigated, as well as the possible underlying mechanisms. Cell viability is measured using an MTT assay. Nitric oxide (NO) release is determined by nitrite assay. Free radical scavenging activity and reactive oxygen species (ROS) production are determined by DPPH reduction assay and DCFH-DA assay. Pretreatment with RV01 (1-30 µm) prior to LPS (1 µg mL-1 ) stimulation decreased NO release and iNOS expression without observable cytotoxicity. RV01 reduced the mRNA levels and secretion of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). RV01 also inhibited LPS-induced ROS production and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activation. Furthermore, RV01 decreases the protein expression of toll-like receptor 4 (TLR4) and inhibits the LPS-induced activation of the mitogen-activated protein kinase (MAPK) and nuclear transcription factor-κB (NF-κB) signaling pathways. Additionally, conditioned medium from microglia co-treated with LPS and RV01 alleviates the death of SH-SY5Y cells induced by conditioned medium from activated N9 microglial cells. Lastly, a mouse neuroinflammation model is further used to confirm the effect of RV01 in vivo. These results show that RV01 suppresses microglia-mediated neuroinflammation and protects neurons from inflammatory damage.

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ALDH2, MAPK, NF-kB, NFkB, NF-κB, NFκB, RV01, RV-01, RV 01, TLR4

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Compound Identifiers

PubChem CID

56837609 ↗

Key Properties

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No computed properties available.

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RV01

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Quality Guarantee

Compound Identifiers

Key Properties

Documents Download

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Protocol Information

RV01 (orb1708629)

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