S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody

SKU: orb1409981

Description

Mouse monoclonal antibody to S100A4

Images & Validation

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Item 1 of 7

Tested Applications	ELISA, IHC, WB
Reactivity	Human, Mouse

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone No.	S100A4/7096
Immunogen	Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
Target	S100A4
Source/Expression System	Bioreactor
Molecular Weight	12kDa
Purification	Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Disclaimer	For research use only

Alternative Names

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S100A4; S100 calcium-binding protein A4; Calvasculin; CAPL; Fibroblast specific protein 1 (FSP1); Leukemia multidrug resistance associated protein; Malignant transformation suppression 1 (MTS1); Metastasin; Placental calcium-binding protein

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S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody

Analysis of Protein Array containing more than 19000 full-length human proteins using S100A4 Mouse Monoclonal Antibody (S100A4/7096). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human lung stained with S100A4 Mouse Monoclonal Antibody (S100A4/7096).

Formalin-fixed, paraffin-embedded human placenta stained with S100A4 Mouse Monoclonal Antibody (S100A4/7096). Inset: PBS instead of primary antibody, secondary only negative control.

Formalin-fixed, paraffin-embedded human spleen stained with S100A4 Mouse Monoclonal Antibody (S100A4/7096). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

SDS-PAGE Analysis Purified S100A4 Mouse Monoclonal Antibody (S100A4/7096). Confirmation of Purity and Integrity of Antibody.

Western Blot Analysis of HeLa lysate using S100A4 Mouse Monoclonal Antibody (S100A4/7096).

Western Blot Analysis of human A549 lysate using S100A4 Mouse Monoclonal Antibody (S100A4/7096).

Quick Database Links

Gene Symbol

S100A4

UniProt

P26447

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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