SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Recombinant Rabbit Monoclonal Antibody

SKU: orb1040884

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Description

Images & Validation

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Item 1 of 5

Tested Applications	ELISA, WB
Dilution range	WB=1:500-2000, ELISA=1:5000-10000
Reactivity	Virus

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	7B2
Immunogen	Recombinant SARS-CoV-2 Nucleocapsid Protein
Target	S
Molecular Weight	46 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein; Ormicron mutant; Ormicron Nucleocapsid Protein; Ormicron N Protein.

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SARS-CoV-2 (2019-nCoV) Nucleocapsid, WT & Ormicron Recognized Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L, E31del, R32del, S33del, R203K, G204R) at 100 ng, Lane 2: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L, E31del, R32del, S33del, R203K, G204R) at 50 ng, Lane 3: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L, E31del, R32del, S33del, R203K, G204R) at 25 ng, Lane 4: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L, E31del, R32del, S33del, R203K, G204R) at 10 ng, Lane 5: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L, E31del, R32del, S33del, R203K, G204R) at 5 ng, Lane 6: Recombinant SARS-CoV-2 N Protein (WT) at 10 ng, Primary: Rabbit Anti-SARS-CoV-2 N protein (orb1040884) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45.3 kD, Observed band size: 50 kD.

Sample: Lane 1: SARS-CoV-2 N Protein (WT) at 10 ng, Lane 2: SARS-CoV-2 N Protein (Del204 & Del215) at 10 ng, Lane 3: SARS-CoV-2 N Protein (R203M & D377Y) at 10 ng, Primary: Rabbit Anti-SARS-CoV-2 N Protein Antibody at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46kD, Observed band size: 46kD.

Sample: Lane 1: SARS-CoV-2 N Protein (WT) at 10 ng, Lane 2: SARS-CoV-2 N Protein (Del204 & Del215) at 2 ng, Lane 3: SARS-CoV-2 N Protein (R203M & D377Y) at 2 ng, Primary: Rabbit Anti-SARS-CoV-2 N Protein Antibody at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46kD, Observed band size: 46kD.

The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.

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Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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