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SARS-CoV-2 6R8A-E11 Human Neutralizing mAb Antibody
SKU: orb1945952
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA |
|---|---|
| Dilution range | Virus neutralization |
| Reactivity | Other |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Monoclonal |
| Isotype | Rabbit IgG1k |
| Clone No. | 6R8A-E11 |
| Purification | Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 1X PBS |
| Concentration | 0.5 mg/mL |
| Disclaimer | For research use only |
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The microtiter wells were coated with recombinant ACE2-Fc protein at 2 μg/mL. The spike trimer protein (96 ng/mL) was mixed with serially diluted antibody 1:1 at RT for 1 hour and added to the wells. Bound spike trimer was detected with horseradish peroxidase conjugated rabbit anti-His tag antibody and the inhibition of binding (IC50 ng/mL) was calculated using Prism 9 software. The experiments were performed in triplicates.
The microtiter wells were coated with recombinant ACE2-Fc protein at 2 μg/mL. The spike trimer protein (96 ng/mL) was mixed with serially diluted antibody 1:1 at RT for 1 hour and added to the wells. Bound spike trimer was detected with horseradish peroxidase conjugated rabbit anti-His tag antibody and the inhibition of binding (IC50 ng/mL) was calculated using Prism 9 software. The experiments were performed in triplicates.
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SARS-CoV-2 6R8A-E11 Human Neutralizing mAb Antibody (orb1945952)
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