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3CL-PRO Antibody
SKU: orb1239090
Description
Research Area
Infectious Disease & Virology
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Virus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody (orb1239090) was raised against a peptide corresponding to 15 amino acids in the center of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein. The immunogen is located within 80-130 amino acids of the SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein. |
| Target | 3CL-PRO |
| Purification | SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−3C-like proteinase, 3CLp, nsp5, Non-structure protein 5, Main protease, Mpro
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ELISA Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein. Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, orb1239090. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein (orb1231584) as coating antigen and the anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
Western Blot Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) recombinant protein (orb1231584). Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5), orb1239090, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL and. Lane 2: 1 µg/mL.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | YP_009742612.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
3CL-PRO Antibody (orb1239090)
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