SARS-CoV-2 (COVID-19) NSP5 Recombinant Protein

SKU: orb1231120

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Description

Research Area

Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Key Properties

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Source	bacteria
Target	ORF1ab
Tag	N-Term GST Cleaved, C-Term 6His Cleaved
Molecular Weight	Mono-Isotopic Mass: 33, 796.64 daltons after tag cleavageAverage Mass: 33, 774.50 daltons after tag cleavage
Protein Sequence	Native Sequence:MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQAmino acids S1 – Q306 (end).Residue S237 of the fusion protein is equivalent to S1 of the native enzyme.The GST tag is located at residues 1 – 220 and the His6 tag is located at residues 545– 550.Has C-terminal 5’ residues of NSP4 (residues 232 – 236) between PreScission site and N-terminus of NSP5 – corresponding to the cleavage site between NSP4 and NSP5 in the polyprotein of the SARS CoV virus to generate an authentic N-terminus during gene expression. The C-terminus encodes for a modified PreScission cleavage site before the His6 tag to generate an authentic C-terminus when cleaved, SGVTFQGP, residues 537 - 544.Protease Cleavage:Modified PreScission - SGVTFQGP, residues 537 - 544
Purity	Cobalt Agarose (0.9)

Storage & Handling

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Storage	Store at -70 °C. Avoid repeated freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35
Concentration	batch dependent
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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SARS-CoV2-NSP5, SARS-CoV-2 NSP5, NSP5 SARS-CoV-2, 3CLPro, 3CL-Pro

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SARS-CoV-2 (COVID-19) NSP5 Recombinant Protein

Quick Database Links

Gene Symbol

ORF1ab

UniProt

P0DTC1

RefSeq:QHD43415.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	QHD43415.1

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