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SARS-CoV-2 (COVID-19) Nucleocapsid Antibody
SKU: orb1239996
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Virus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (orb1239996) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Nucleocapsid protein. The immunogen is located within 350-400 amino acids of SARS-CoV-2 (COVID-19) Nucleocapsid protein. |
| Target | N |
| Purification | SARS-CoV-2 (COVID-19) Nucleocapsid Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) Nucleocapsid Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−SARS-CoV-2 (COVID-19) Nucleocapsid Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Nucleocapsid protein, N protein, Nucleocapsid Phosphoprotein
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Western Blot Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein, orb1231585. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid, orb1239996, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and Lane 2: 2 µg/mL.
Western Blot Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein. Loading: 50 ng per lane of SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid, orb1239996, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL and Lane 3: 2 µg/mL.
ELISA Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid antibody, orb1239996 (1 µg/mL). A direct ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.
ELISA Validation of SARS-CoV-2 Nucleocapsid Antibody Pair. A sandwich ELISA was performed using the anti-SARS-COV-2 Nucleocapsid antibodies orb1239996 (10 µg/mL) as the capture antibody. Biotin-labled anti-SARS-COV-2 Nucleocapsid antibodies orb1239067 (1 µg/mL) and streptavidin-HRP (0.1 µg/mL) were used for detection. Detection range is from 3 ng to 200 ng (SARS-CoV-2 Nucleocapsid Protein, orb1231585).
Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, orb1273021 and orb1239996. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (orb1273021, 5 ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (orb1231585), and the anti-SARS-CoV-2 Nucleocapsid antibody (orb1239996, 1 ug/ml) as the detection antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 6.9 ng.
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WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SARS-CoV-2 (COVID-19) Nucleocapsid Antibody (orb1239996)
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